Journal: Heliyon

Article Title: Inhibition of CTLA-4 accelerates atherosclerosis in hyperlipidemic mice by modulating the Th1/Th2 balance via the NF-κB signaling pathway

doi: 10.1016/j.heliyon.2024.e37278

Figure Lengend Snippet: The anti-CTLA-4 antibody promotes CD4 + T-cell infiltration and T-cell differentiation into Th1 cells in the aortic root. (A) GO enrichment analysis showing the significantly enriched biological processes in hyperlipidemic mice administered the anti-IgG antibody or anti-CTLA-4 antibody. n = 3 per group. (B) GSEA of RNA-seq data showing the enrichment of “regulation of Th1-type immune response” and “Th1 cell differentiation” in hyperlipidemic mice administered the anti-IgG antibody or anti-CTLA-4 antibody. NES: normalized enrichment score; FDR: adjusted p value. (C) Representative immunofluorescence staining of CD4 (red), α-SMA (green), and DAPI (blue) in mice in the indicated groups. The square represents the colocalization of CD4 with the smooth muscle marker α-SMA. The scale bars are 100 μm and 20 μm. (D) Quantitative analysis of CD4 fluorescence intensity in aortic roots in the indicated groups. The data are presented as the means ± SEM (n = 6). (E) Flow cytometric analysis of the distributions of CD4 + IFN-γ + T cells and CD4 + IL-4 + T cells in peripheral blood in the indicated groups. (F) Relative quantification of the percentages of CD4 + IFN-γ + T cells and CD4 + IL-4 + T cells among the sorted CD4 + T cells. The data are presented as the means ± SEM (n = 5). (G) The Th1/Th2 ratio was estimated by determining the ratio of CD4 + IFN-γ + IL-4 − cells to CD4 + IFN-γ − IL-4 + cells. The data are presented as the means ± SEM (n = 5). (H) Quantitative results were obtained by real-time PCR showing the relative mRNA expression of IFN-γ and IL-4 in the indicated groups. The data are presented as the means ± SEM (n = 6). *P < 0.05 vs . the anti-IgG group. (I – J) Quantitative analyses demonstrating the number of CD4 + IFN-γ + T cells and CD4 + IL-4 + T cells in aortic roots from each indicated experimental group. The data are presented as the means ± SEM (n = 4). *P < 0.05 vs . the anti-IgG group.

Article Snippet: One hundred microliters of mouse peripheral blood were collected in isotonic RPMI 1640 complete culture medium (R8758, Sigma-Aldrich) and stimulated with 2 μL of leukocyte activation cocktail (550583, BD Biosciences) in 5 % CO 2 at 37 °C for 4 h. Then, the cells were treated with anti-mouse CD3 (APC-Cy7-CD3, 1:200, 557596, BD Biosciences) and anti-mouse CD4 (BV510-CD4, 1:200, 563106, BD Biosciences) for 30 min and lysed in red blood cell lysis solution (130-094-183, Miltenyi Biotec) for 10 min. After being treated with fixation/permeabilization concentrate at room temperature for 1 h, total T lymphocytes were further stained with anti-mouse IFN-γ (FITC-IFN-γ, 1:100, 554411, BD Biosciences) and anti-mouse IL-4 (PE-IL-4, 1:100, 554435, BD Biosciences) for 30 min. Isotype controls were used for compensation and to confirm antibody specificity.

Techniques: Cell Differentiation, RNA Sequencing, Immunofluorescence, Staining, Marker, Fluorescence, Quantitative Proteomics, Real-time Polymerase Chain Reaction, Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Suppression of ongoing experimental autoimmune encephalomyelitis by neutralizing the function of the p28 subunit of IL-27.

doi: 10.4049/jimmunol.173.10.6465

Figure Lengend Snippet: FIGURE 1. DNA vaccination-based anti-IL-27 Abs are highly specific. The Western blot shows that our DNA vaccination-based anti-IL-27 Ab binds mouse IL-27 p28 (lane 1; 27 kDa), but not recombinant mouse IL-18, IL-12, or TNF- (lanes 2, 3, and 4, respectively). A, Coomassie Blue staining verifies the appearance of each cytokine on the loaded gel. B, Western blot showing that of these cytokines, our DNA vaccination-based anti-IL-27 Ab binds only mouse IL-27 p28. These Ab also bound natural mouse IL-27 (verified by sequencing) from supernatant of activated MOGp35–55-specific cultured primary draining lymph node cells (not shown).

Article Snippet: Our recombinant mouse IL-27 p28, produced as described above, and commercially available recombinant mouse IL-18, IL-12, and TNF- (R&D Systems) were each subjected to Western blot analysis according to the protocol described in detail previously (29, 32), with the minor modification of using a 12% (rather than 8%) running gel.

Techniques: Western Blot, Recombinant, Staining, Sequencing, Cell Culture

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Suppression of ongoing experimental autoimmune encephalomyelitis by neutralizing the function of the p28 subunit of IL-27.

doi: 10.4049/jimmunol.173.10.6465

Figure Lengend Snippet: FIGURE 2. Anti-p28 Abs suppresses ongoing severe EAE. A, Four groups of 10 mice each were subjected to MOGp35–55-induced EAE. Beginning at the onset of disease (day 17), these mice were repeatedly (every other day) administered 100 g/mouse of anti-p28 Ab (f), IgG obtained from naive Lewis rats (Œ), or PBS (E). An observer blind to the experimental procedure scored EAE daily. The experiment summarized in Fig. 2 shows the results of one of three experiments performed under similar experimental conditions, with similar results. The mean maximal score SE represents six mice per group. The other four mice were killed on day 30 and subjected to histological evaluation (see Fig. 3). B, Five groups of six mice each were subjected to MOGp35–55-induced EAE. Beginning at the onset of disease (day 17), these mice were repeatedly (every other day) administered 100 g of anti-p28 Ab/mouse (f), anti-IL-18 Ab (F), anti-IL-1 Ab (), IgG obtained from Lewis rats previously subjected to an empty plasmid administration (Œ), or PBS (E). An observer blind to the experimental procedure scored EAE daily. Results are shown as the mean maximal score SE of six mice per group. C, Three groups of six mice each were subjected to induction of transferred EAE. Beginning at the onset of disease (day 5), these mice were repeatedly (every other day) administered 100 g of anti-p28 Ab/mouse (f), IgG obtained from naive Lewis rats (Œ), or PBS (E). An observer blind to the experimental procedure scored EAE daily. Results are shown as mean maximal score SE of six mice per group.

Article Snippet: Our recombinant mouse IL-27 p28, produced as described above, and commercially available recombinant mouse IL-18, IL-12, and TNF- (R&D Systems) were each subjected to Western blot analysis according to the protocol described in detail previously (29, 32), with the minor modification of using a 12% (rather than 8%) running gel.

Techniques: Plasmid Preparation

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Suppression of ongoing experimental autoimmune encephalomyelitis by neutralizing the function of the p28 subunit of IL-27.

doi: 10.4049/jimmunol.173.10.6465

Figure Lengend Snippet: FIGURE 4. The beneficial effect of anti-IL-27 is dependent on the con- tinuing administration of protective Abs. Three groups of six mice each were subjected to active induction of EAE. Beginning 1 day after the onset of disease (day 17), these mice were treated with either a single dose of anti-p28 Ab (100 g/mouse; E) or with repeated administration (every other day) of this Ab (Œ) or PBS (f). An observer blind to the experi- mental procedure scored EAE daily. Results are shown as the mean max- imal score SE of six mice per group.

Article Snippet: Our recombinant mouse IL-27 p28, produced as described above, and commercially available recombinant mouse IL-18, IL-12, and TNF- (R&D Systems) were each subjected to Western blot analysis according to the protocol described in detail previously (29, 32), with the minor modification of using a 12% (rather than 8%) running gel.

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Suppression of ongoing experimental autoimmune encephalomyelitis by neutralizing the function of the p28 subunit of IL-27.

doi: 10.4049/jimmunol.173.10.6465

Figure Lengend Snippet: FIGURE 3. Anti-IL-27 therapy reduces the histological score of EAE. Histological evaluation was conducted 30 days after disease induction. Lumbar spinal cord samples from naive mice or from EAE mice treated with PBS, IgG from naive mice, or anti-IL-27 p28 Abs were subjected to histological analysis (nine sections each group). The arrowheads point to the parenchymal mononuclear cell infiltration. The scale for mononuclear cell infiltration used was: 0, no mononuclear cell infiltration; 1, one to five perivascular lesions per section with minimal parenchymal infiltration; 2, five to 10 perivascular lesions per section with parenchymal infiltration; and 3, 10 perivascular lesions per section with extensive parenchymal infiltration. The mean histological score SE was calculated for each group.

Article Snippet: Our recombinant mouse IL-27 p28, produced as described above, and commercially available recombinant mouse IL-18, IL-12, and TNF- (R&D Systems) were each subjected to Western blot analysis according to the protocol described in detail previously (29, 32), with the minor modification of using a 12% (rather than 8%) running gel.

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Suppression of ongoing experimental autoimmune encephalomyelitis by neutralizing the function of the p28 subunit of IL-27.

doi: 10.4049/jimmunol.173.10.6465

Figure Lengend Snippet: FIGURE 5. Protective administration of anti-IL-27 Abs decreases in vivo polarization of CD4 T cells into Th1 and suppresses IFN- production by Ag-specific T cells. C57BL/6 mice (three per group) were subjected to active induction of EAE and then to repeated administration (days 12, 14, and 16) of anti-IL-27 p28 Abs (100 g), PBS, or normal rat IgG. On day 17 cervical lymph node cells (that drain the autoimmune site) were subjected to intracellular staining of IL-4 and IFN-. A, FACS analysis of CD4 T cells in this experiment. This experiment represents results obtained in three different independent experiments with very similar data. Subsequently, cervical lymph node T cells from these mice were cultured in the presence of 100 M MOGp35–55. After 72 h of stimulation, supernatants were assayed for the protein level of IFN- (B) and IL-4 (not shown). This experiment represents results obtained in three different independent experiments with very similar data.

Article Snippet: Our recombinant mouse IL-27 p28, produced as described above, and commercially available recombinant mouse IL-18, IL-12, and TNF- (R&D Systems) were each subjected to Western blot analysis according to the protocol described in detail previously (29, 32), with the minor modification of using a 12% (rather than 8%) running gel.

Techniques: In Vivo, Staining, Cell Culture

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Suppression of ongoing experimental autoimmune encephalomyelitis by neutralizing the function of the p28 subunit of IL-27.

doi: 10.4049/jimmunol.173.10.6465

Figure Lengend Snippet: FIGURE 6. Neutralizing the function of IL-27 reduces IFN- produc- tion by IFN--producing T cells. A, C57BL/6 mice (three per group) were subjected to active induction of EAE and then to repeated administration (days 3 and 6) of 100 g of anti-IL-27 p28 Abs (group 3), PBS (group 2), or normal rat IgG (group 1). On day 9, spleen cells were subjected to spot ELISA as previously described (38). A, Relative number of positive spots per 107 cultured cells. The average size of positive spots was analyzed. B, The MOGp33–55-specific CD4 T cell line was cultured with or without 100 M MOGp33–55. Cultured cells were supplemented with anti-IL-27 Abs at a final concentration of 10 g/ml (), normal rat IgG (f), or PBS (E). After 60 h of incubation, cells were plates in spot ELISA plates for an additional 24 h for the detection of IFN--positive spots (38). Number of positive spots (y-axis) and spot sizes (x-axis; logarithmic scale) determined as previously described (46).

Article Snippet: Our recombinant mouse IL-27 p28, produced as described above, and commercially available recombinant mouse IL-18, IL-12, and TNF- (R&D Systems) were each subjected to Western blot analysis according to the protocol described in detail previously (29, 32), with the minor modification of using a 12% (rather than 8%) running gel.

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Concentration Assay, Incubation

Journal: Disease Models & Mechanisms

Article Title: Evaluation of human dermal fibroblasts directly reprogrammed to adipocyte-like cells as a metabolic disease model

doi: 10.1242/dmm.030981

Figure Lengend Snippet: Characterisation of direct reprogrammed adipocyte-like cells. (A) Quantitative RT-PCR analysis of expression of marker genes of white adipocytes ( FABP4 , ADIPOQ , CEBPA and SLC2A4 ) at four different differentiation time points. (B) Western blot analysis of key proteins in adipocyte-like cells during direct reprogramming up to day 18, using antibodies as indicated. (C) Adiponectin secretion from direct reprogrammed adipocyte-like cells in 48-h culture medium was determined with DELFIA. (D) Downregulation of PPARγ2 and then INSR expression after removal of DOX from the culture medium. (E) Delipidation was also observed in reprogrammed adipocyte-like cells. Images were taken 10 days after DOX withdrawal (left) or with DOX included in the culture medium throughout (right). Scale bars: 100 µm. (F) Glucose uptake assay. (G) Lipolysis assay of direct reprogrammed adipocyte-like cells treated with isoprotenerol and/or IBMX. Data are mean±s.e.m. from three independent experiments (*** P <0.001, Student's t -test).

Article Snippet: Blots were blocked in TBST (50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.1% Tween 20) containing 5% milk or BSA (Sigma-Aldrich) and probed overnight at 4°C with the following antibodies: anti-PPARγ (sc-7196, Santa Cruz Biotechnology, 1:500 dilution), anti-adiponectin (ab13881, Abcam, 1:1000 dilution), anti-Glut4 (ab654, Abcam, 1:4000 dilution), anti-C/EBPa (#2295, Cell Signaling Technology, 1:1000 dilution), anti-insulin receptor β (sc-711, Santa Cruz Biotechnology, 1:500 dilution), anti-IGF1 receptor (sc-713, Santa Cruz Biotechnology, 1:500 dilution), anti-calnexin (ab75801, Abcam, 1:10,000 dilution), anti-aP2 (sc-18661, Santa Cruz Biotechnology, 1:5000 dilution), anti-lamin A/C (sc-20681, Santa Cruz Biotechnology, 1:500 dilution), anti-perilipin (#3467s Cell Signaling Technology, 1:1000 dilution) and anti-lamin R482W (SAB4200422, Sigma-Aldrich, 1:5000 dilution).

Techniques: Quantitative RT-PCR, Expressing, Marker, Western Blot

Journal: Disease Models & Mechanisms

Article Title: Evaluation of human dermal fibroblasts directly reprogrammed to adipocyte-like cells as a metabolic disease model

doi: 10.1242/dmm.030981

Figure Lengend Snippet: Direct reprogramming of dermal fibroblasts derived from patients with metabolic diseases caused by mutations on PPARγ, insulin receptor, lamin and BSCL2. (A) Oil Red O staining showing that the four patient cell lines (SIR1-SIR4) were all successfully converted into adipocyte-like cells using our direct reprogramming protocol, as schematised in Fig. 1 . Scale bars: 200 µm. (B) Quantitative RT-PCR analysis of the expression of white adipocyte marker genes. (C) Western blot analysis of key proteins in undifferentiated control cells (DOX−) and reprogrammed adipocyte-like cells (DOX+). Sequencing confirmation of mutations on PPARG and BSCL2 genes is shown for SIR1 and SIR4, respectively. (D) Adiponectin secretion from direct reprogrammed adipocyte-like cells in 48-h culture medium was determined with DELFIA. Data are mean±s.e.m. from three independent experiments (*** P <0.001, Student's t -test).

Article Snippet: Blots were blocked in TBST (50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.1% Tween 20) containing 5% milk or BSA (Sigma-Aldrich) and probed overnight at 4°C with the following antibodies: anti-PPARγ (sc-7196, Santa Cruz Biotechnology, 1:500 dilution), anti-adiponectin (ab13881, Abcam, 1:1000 dilution), anti-Glut4 (ab654, Abcam, 1:4000 dilution), anti-C/EBPa (#2295, Cell Signaling Technology, 1:1000 dilution), anti-insulin receptor β (sc-711, Santa Cruz Biotechnology, 1:500 dilution), anti-IGF1 receptor (sc-713, Santa Cruz Biotechnology, 1:500 dilution), anti-calnexin (ab75801, Abcam, 1:10,000 dilution), anti-aP2 (sc-18661, Santa Cruz Biotechnology, 1:5000 dilution), anti-lamin A/C (sc-20681, Santa Cruz Biotechnology, 1:500 dilution), anti-perilipin (#3467s Cell Signaling Technology, 1:1000 dilution) and anti-lamin R482W (SAB4200422, Sigma-Aldrich, 1:5000 dilution).

Techniques: Derivative Assay, Staining, Quantitative RT-PCR, Expressing, Marker, Western Blot, Sequencing

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Mammalian adiponectin co-localizes with the mosquito midgut. (A) Following a sugar meal or blood meal, mRNA from the mosquito midgut, ovary, or fat body was collected. The expression level of the adiponectin receptor was determined by RT-PCR. Each dot in the midgut and ovary groups represents a pooled sample from two mosquitoes. Each dot in the fat body group represents a pooled sample from four mosquitoes. (Mean ± S.D.) (B) Murine adiponectin bound to mosquito midguts. His-tag murine adiponectin or control protein (BSA) was incubated with the mosquito midgut, and the presence of adiponectin in the midgut was determined by confocal microscopy.

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation, Confocal Microscopy

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Lack of adiponectin in the incoming blood meal increases infection of Anopheles gambiae with Plasmodium berghei . (A) P. berghei -infected RBCs were collected from Adipoq -/- mice, and then equal amounts of plasma from Adipoq -/- mice or wild-type mice were mixed with the infected RBCs. Mosquitoes were fed on blood lacking adiponectin or wild-type plasma by membrane feeding. Two days after the blood meal, the midguts were collected, and the expression levels of the adiponectin receptor ( A ) and the burden of Plasmodium were determined by RT-qPCR ( B ). (C) Fourteen days after the blood meal, the midguts of mosquitoes were collected and the burden of Plasmodium was determined by RT-qPCR. (D) Mosquitoes were injected with dsAdpR ( AdpR knockdown, AdpR KD), or dsGluc (control). Two days after ds RNA injection, the mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, the midguts were collected and the expression levels of adiponectin receptor ( D ) and the burden of Plasmodium were determined by RT-PCR ( E ). (F) Fourteen days after the blood meal, the midguts of mosquitoes were collected, and the burden of Plasmodium was determined by RT-PCR. Each dot represents one mosquito. (Median ± interquartile range, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Infection, Membrane, Expressing, Quantitative RT-PCR, Injection, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Gene expression analysis reveals that lipophorin (AGAP001826) is regulated by the adiponectin receptor. Mosquitoes were injected with dsAdpR ( AdpR knockdown, KD) or dsGluc (control, Ctrl). Two days after ds RNA injection, the mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, each midgut was collected, and RNA from each midgut was isolated for RNA-seq analysis. (A) A hierarchical clustering of differentially expressed genes was generated by comparing data from AdpR knockdown and control mosquitoes. (B) Volcano plots showing significant changes in gene expression between AdpR knockdown and control mosquitoes. Dispersion graph of –10*log10( P value) (y-axis) against the log2(fold change) (x-axis) corresponding to the genes by their differential expression. (C) RT-qPCR validation of gene expression in the mosquito midgut after injection with ds AdpR (Adiponectin knockdown, AdpR KD) or ds Gluc (control). Two days after ds RNA injection, mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, the midguts were collected for RT-qPCR. (D) RT-qPCR validation of expression in mosquito midguts 2 days after feeding on adiponectin knockout (Adipoq -/- ) or wild-type P. berghei -infected mice. Each dot represents one mosquito. (Median ± interquartile range, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Expressing, Injection, Infection, Isolation, RNA Sequencing Assay, Generated, Dispersion, Quantitative RT-PCR, Knock-Out, MANN-WHITNEY

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Adiponectin signaling affects Plasmodium infection in mosquitoes through the lipophorin pathway. Mosquitoes were injected with ds AdpR ( AdpR knockdown), ds Lp ( lipophorin knockdown), both ds AdpR and ds Lp, or dsGluc (control). Two days after injection, the mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, the midguts were collected, and the expression levels of adiponectin receptor ( A ) and lipophorin ( B ) were determined by RT-qPCR. (C) Hemolymph from 10 knockdown or control mosquitoes was collected, and the levels of lipophorin (Lp) and vitellogenin (Vg) were determined by SDS-PAGE gel. (D) The Plasmodium burden was determined by RT-qPCR. Each dot represents one mosquito. (Median ± interquartile range, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Infection, Injection, Expressing, Quantitative RT-PCR, SDS Page, MANN-WHITNEY

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Silencing of adiponectin signaling affects the oocyst number and size in mosquitoes. Mosquitoes were injected with ds AdpR ( AdpR knockdown) or dsGluc (control). Two days after injection, the mosquitoes were fed on GFP-expressing P. berghei -infected mice. (A) Seven days after the blood meal, the midguts were collected. The number of oocysts per midgut was determined by the GFP signals using EVOS fluorescence microscopy. The dot blots represent the number of oocysts from each group of infected midguts. (Median ± interquartile range (IQR), P < 0.05 using the Mann-Whitney U-test.) The pie charts represent the percentage of infected mosquitoes in each group ( P < 0.05 using the Chi-square test). (B) The size of oocysts in each group was determined by GFP signals using EVOS fluorescence microscopy. (Median ± IQR, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Injection, Expressing, Infection, Fluorescence, Microscopy, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Clinicopathological Features and Prognosis of Papillary Thyroid Microcarcinoma for Surgery and Relationships with the BRAF V600E Mutational Status and Expression of Angiogenic Factors

doi: 10.1371/journal.pone.0167414

Figure Lengend Snippet: Information of the primers used in Q-PCR.

Article Snippet: Next, the slides underwent antigen retrieval in sodium citrate solution in a microwave oven, were blocked with 5% bovine serum albumin for 30 min, and were incubated overnight with a rabbit anti-human PEDF primary polyclonal antibody at a 1:100 dilution (Boster Bio-Engineering, China) at 4°C.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Clinicopathological Features and Prognosis of Papillary Thyroid Microcarcinoma for Surgery and Relationships with the BRAF V600E Mutational Status and Expression of Angiogenic Factors

doi: 10.1371/journal.pone.0167414

Figure Lengend Snippet: Clinicopathologic characteristics of 251 patients with papillary thyroid carcinomas.

Article Snippet: Next, the slides underwent antigen retrieval in sodium citrate solution in a microwave oven, were blocked with 5% bovine serum albumin for 30 min, and were incubated overnight with a rabbit anti-human PEDF primary polyclonal antibody at a 1:100 dilution (Boster Bio-Engineering, China) at 4°C.

Techniques: Mutagenesis

Journal: PLoS ONE

Article Title: Clinicopathological Features and Prognosis of Papillary Thyroid Microcarcinoma for Surgery and Relationships with the BRAF V600E Mutational Status and Expression of Angiogenic Factors

doi: 10.1371/journal.pone.0167414

Figure Lengend Snippet: Clinicopathological characteristics in papillary thyroid carcinomas according to tumor size.

Article Snippet: Next, the slides underwent antigen retrieval in sodium citrate solution in a microwave oven, were blocked with 5% bovine serum albumin for 30 min, and were incubated overnight with a rabbit anti-human PEDF primary polyclonal antibody at a 1:100 dilution (Boster Bio-Engineering, China) at 4°C.

Techniques: Mutagenesis

Journal: PLoS ONE

Article Title: Clinicopathological Features and Prognosis of Papillary Thyroid Microcarcinoma for Surgery and Relationships with the BRAF V600E Mutational Status and Expression of Angiogenic Factors

doi: 10.1371/journal.pone.0167414

Figure Lengend Snippet: Differences in the clinicopathological characteristics between high-risk PTMC and LPTC.

Article Snippet: Next, the slides underwent antigen retrieval in sodium citrate solution in a microwave oven, were blocked with 5% bovine serum albumin for 30 min, and were incubated overnight with a rabbit anti-human PEDF primary polyclonal antibody at a 1:100 dilution (Boster Bio-Engineering, China) at 4°C.

Techniques: Mutagenesis, Expressing

Journal: Immunity

Article Title: T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis

doi: 10.1016/j.immuni.2022.12.010

Figure Lengend Snippet:

Article Snippet: InVivoMAb rat IgG2a (RG7/1.30) , BioXcell , Cat# BE0251; RRID: AB_2687732.

Techniques: Purification, Control, Virus, Recombinant, Red Blood Cell Lysis, Transfection, Flow Cytometry, DNA Extraction, Gel Extraction, Plasmid Preparation, cDNA Synthesis, Cell Differentiation, Staining, Cell Isolation, Conjugation Assay, Sequencing, CRISPR, Retroviral, Software